Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MMLDKDSVKAIDVQTASLQSYISSPEKQKSLYKRTLFVVSISQIFGGAGLAAGVTVGALIAQQMLGTDAFAGLPSALFTLGSAGSALIVGRLSQRYGRRTGLSAGFMIGGLGAIGVIMAAIINSIFLLFISLLIYGAGTAT-NLQARYAGTDLANHKQRATAVSITMVFTTFGAVAGPSL-------------VNVMGDFALSIGVPSLAGPFILAAAAYMLAGVVLFIMLRPDPLVIARTIEAANEEPGDKGHLAATEHT-ENKKGIIVGATVMVLTQIVMVAIMTMTPVHMRHH--GHDLGAVGLVIGFHIGAMYLPSLVTGVLVDRLGRTAMAISSGTTLLLAGVIAAFAPGESMILLVIALSLLGLGWNFGLISGTALIVDSTDTATRAKTQGTVDVLIALSGAAGGALSGMIVAGSSYLALSLIGGILSLLLIPVVVWSRGR- 3O7Q Chain:A ((22-435)) ----------------------------RSYIIPFALLCSLFFLWAVANNLNDILLPQFQQAFTL-TNFQAGLIQSAFYFGYFIIPIPAGILMKKLSYKAGIITGLFLYALGAALFWPAAEIMNYTLFLVGLFIIAAGLGCLETAANPFVTVLGPESSGHFRLNLAQTFNSFGAIIAVVFGQSLILSNVPHQSQDVLDKMSPEQLSAYKHSLVLSVQTPYMIIVAIVLLVALLIMLTKFPALQSDNHSDAKQGSFSASLSRLARIRHWRWAVLAQFCYVGAQTACWSYLIRYAVEEIPGMTAGFAANYLTGTMVCFFIGRFTGTWLISRFAPHKVLAAYALIAMALCLISAFAGG-----HVGLIALTLCSAFMSIQYPTIFSLGIKNLGQDTKYGSSFIVMTIIGGGIVTPVMGFVSDAAGNIPTAELIPALCFAVIFIFARFRSQT

General information: TITO was launched using: RESULT: Template: 3O7Q.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1965 -292473 -148.84 -738.57 target 2D structure prediction score : 0.56 Monomeric hydrophicity matching model chain A : 0.70 3D Compatibility (PKB) : -148.84 2D Compatibility (Sec. Struct. Predict.) : 0.56 1D Compatibility (Hydrophobicity) : 0.70 QMean score : 0.232

(partial model without unconserved sides chains): PDB file : Tito_3O7Q.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3O7Q-query.scw PDB file : Tito_Scwrl_3O7Q.pdb: