Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKTKTAFMAILFSLITVLSACGAGSQTTGAGQKKVQTITVGTGTQFPNICFIDEKGDLTGYDVELIKELDKRLPHYKFTFKTMEFSNLLVSLGQHKVDIVAHQMEKSKEREKKFLFNKVAYNHFPLKITVLQNNDTIRGIEDLKGKRVITSATSNGALVLKKWNEDNGRPFEI--AYEGQGANETANQLKSGRADATISTPFAVDFQNKTST-IKEKTVGNVLSNAKVY-FMFNKNEQTLSDDIDKALQEIIDDGTLKRLSLKWLGDDYSKEQY 4EQ9 Chain:A ((7-238)) ----------------------------------KKEIIVATNGSPRPFIYEE-NGELTGYEIEVVRAIFKDSDKYDVKFEKTEWSGVFAGLDADRYNMAVNNLSYTKERAEKYLYAA-PIAQNPNVLVVKKDDSSIKSLDDIGGKSTEVVQATTSAKQLEAYNAEHTDNPTILNYTKA-DFQQIMVRLSDGQFDYKIFDKIGVETVIKNQGLDNLKVIELPSDQQPYVYPLLAQGQDELKSFVDKRIKELYKDGTLEKLSKQFFGDTY-----

General information: TITO was launched using: RESULT: Template: 4EQ9.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1108 -25474 -22.99 -111.73 target 2D structure prediction score : 0.53 Monomeric hydrophicity matching model chain A : 0.71 3D Compatibility (PKB) : -22.99 2D Compatibility (Sec. Struct. Predict.) : 0.53 1D Compatibility (Hydrophobicity) : 0.71 QMean score : 0.422

(partial model without unconserved sides chains): PDB file : Tito_4EQ9.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4EQ9-query.scw PDB file : Tito_Scwrl_4EQ9.pdb: