Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSVQEHKQEAPDIVRCKVITVSDT--RTDETDKSGKLMISFLEEAGHHIAAYEIVKDEKDALQRSVLAGCMDEQTDAVLLNGGTGIADRDVTIEAITPLFSKELPGFGEIFRMLSYTEDIGSAAIMSRATAGVIQHTAVFSTPGSSGAVKLAMNKLIIPELAHVVREIRKDK 2IS8 Chain:A ((1-156)) ------------MFRVGILTVSDKGFRGERQDTTHLAIREVLAGGPFEVAAYELVPDEPPMIKKVLRLWADREGLDLILTNGGTGLAPRDRTPEATRELLDREVPGLAELMRLVGLR--KTPMAALSRGVAGVRGRTLILNLPGSPKGARESLE-AVLPVLPHALSLVTGK-

General information: TITO was launched using: RESULT: Template: 2IS8.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 854 871 1.02 5.66 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain A : 0.78 3D Compatibility (PKB) : 1.02 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.78 QMean score : 0.472

(partial model without unconserved sides chains): PDB file : Tito_2IS8.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2IS8-query.scw PDB file : Tito_Scwrl_2IS8.pdb: