Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKKQCVAMLLAGGKGSRLSGLTKNMAKPAVSFGGKYRIIDFTLSNCSNSGIDTVGILTQYQPLELNSYIGIGS-AWDLDRYNGGVTVLPPYAESSEVKWYKGTASSIYENLNYLNQYDPEYVLILSGDHI-YK-MDYGKMLDYHIEKKADVTISVIEVGWEEASRFGIMKANPDGTITHFDEKPKF----PKSNLASMGIYIFNWPLLKQYLEMDDQNPYSSHDFGKDIIPLLLEEKKKLSAYPFK--GYWKDVGTVQSLWEANMDLLKEDSEL------KLFER-KWKIYSVNP------------------NQPPQFISSDAQVQ----------------DSLVNEGCVV--------------------YGNVSHSVLFQGVTVGKHTTVTSSVIMPDVTIGEHVVI--------ENAIVPNGMVLPDGAVIRSEKDIEEVLLVSEEFVEKELI 4G3Q Chain:A ((12-444)) ---DTAVLVLAAGPGTRMRS---DTPKVLHTLAGR-SMLSHVLHAIAKLAPQRLIVVLGHDHQRIAPLVGELADTLG-----RTIDVALQ-D------RPLGTGHAVLCGLSALPDDYAGNVVVTSGDTPLLDADTLADLIATHRAVSAAVTVLTTTLDD--PFGYGRILRTQDHEVMAIVEQTDATPSQREIREVNAGVYAFDIAALRSALSRLSSNNAQQELYLTDVIAILRSDGQTVHASHVDDSALVAGVNNRVQLAELASELNRRVVAAHQLAGVTVVDPATTWIDVDVTIGRDTVIHPGTQLLGRTQIGGRCVVGPDTTLTDVAVGDGASVVRTHGSSSSIGDGAAVGPFTYLRPGTALGADGKLGAFVEVKNSTIGTGTKVPHLTYVGDADIGEYSNIGASSVFVNYDGTSKRRTTVGSHVRTGSDTMFVAPVTIGDGAYTGAGTVV----

General information: TITO was launched using: RESULT: Template: 4G3Q.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2070 -68533 -33.11 -193.05 target 2D structure prediction score : 0.56 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : -33.11 2D Compatibility (Sec. Struct. Predict.) : 0.56 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.423

(partial model without unconserved sides chains): PDB file : Tito_4G3Q.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4G3Q-query.scw PDB file : Tito_Scwrl_4G3Q.pdb: