Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MRVFVARQPIFNR-KEQVVAYELLYRES-EENVYSA-K--D------GDQATTDLVINSFLNIGIEKLTEGKRCFVNFTESLM-FSNLP----------TSFNPKQLVIEILEDI--PITPALISRCKELKKMGYMLALDDFYAINPQDEDLLEKLMSY-IDILKIDFLKTTRM--------ERRTILQTYGCRGLIFLAEKVETRKEYKQAVQDGFQLFQGYFFSEPRIISGHDLSTHFYSYYELLNELSKEQPNIKRVTEYIERDLSLSYQILKFLNSSHSRLSQKIESIQQAIMLLGFNEIKRWIYILSFKDLSRKGHSSKHEIIKISLIRAKLCELLARKTSRPQPASYMLIGMFSLIDTLLHREIEEIVQELPLKDEVGQALLGHQNDYYQMLELVKLIESNNWDTCSELGNQLDKEEAYECYLEALEWCHNLMDAK 3GFZ Chain:A ((176-398)) -PCQFALQAIVEPAKKRVSSFEALIRSPTGG-SPVEMFAAIAAEDRYRFDLESKAYAFALAG---QLPLGKHQLAINLLPGSLYHHPDAVGWLMDSLLAAGLRPDQVLIEVTETEVITCFDQFRKVLKALRVAGMKLAIDDFGA-GYSG---LSLLTRFQPDKIKVDAELVRDIHISGTKQAIVASVVRCCEDLGITVVAEGVETLEEWCWLQSVGIRLFQGFLFSRPCLNG------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 3GFZ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 864 -21650 -25.06 -113.95 target 2D structure prediction score : 0.50 Monomeric hydrophicity matching model chain A : 0.58 3D Compatibility (PKB) : -25.06 2D Compatibility (Sec. Struct. Predict.) : 0.50 1D Compatibility (Hydrophobicity) : 0.58 QMean score : 0.433

(partial model without unconserved sides chains): PDB file : Tito_3GFZ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3GFZ-query.scw PDB file : Tito_Scwrl_3GFZ.pdb: