Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ---MVTRITGLASGMDIDDIVSKLMQTERAPL-----DKLTQKKQTLEWQRDSYREVNSKIKELQDYMSKNTL---TYPSTYQSKTVTSSNESVLTATGSVSAPNSSSTVEVASLATAATYKANNYTGYTQGDYNLAFNVVAPGETTAKTVNISVTSADTIDNVISKLNSSDLGVSAFKDKIWNGTEYVETIAFSSKATGAGGSIQAADSATADFMSGQLGFSLDADNKLTAYKEGTNAKVTINGFEMEKLTNNFTVNGVTYSIKNTTAATGPVTTSVSTDVDGI---YNQIKEFVDKYNELVDSLNEKLKEEKYRDYTPLTSEQKEAMSDKEVELWEEKAKSGLLRNDSSISTGTNQMRTDFYTQVNADGKTYQLTEFGITTSSAYQLRGHLEINEEKLKAKIAEDPQGVANLFTSGTNDSNYSDKGIMKRITNTLRSTVKSIEAKAGNSTMGASSYSIGKNLNSISTEITDMQDRLNTIENRYYSKFSAMDSAIQKMNEQASYLSQLLVQ 4O2X Chain:A ((5-483)) EGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTWDAVRYNGKLIAYP---IAVEALSLIYNKDLLPNPPKTWEE---------IPALDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYE--NGKYDIKDVGVDN-----AGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFLENYL-LTDEGLEAVNKDKPLGAVALKSYEEELAKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQT--------VDEALAAAQTNAAANLEKIKKLRNVITSAWKVILY---NDDIHN----FTYVTDVIVKVVGQIS-KAKAHTITVEAHSTG----------------QALILSTWKSKAEKYCQELQQNGLTVSIIHES---

General information: TITO was launched using: RESULT: Template: 4O2X.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2452 158515 64.65 357.82 target 2D structure prediction score : 0.49 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : 64.65 2D Compatibility (Sec. Struct. Predict.) : 0.49 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.167

(partial model without unconserved sides chains): PDB file : Tito_4O2X.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4O2X-query.scw PDB file : Tito_Scwrl_4O2X.pdb: