Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKEEIIERFTTYVKVDTQSDESVDTCPSTPGQLTLGNMLVDELKSIGMQDAAIDENGYVMATLPSNTEKDVPTIGFLAHVDTATDFTGKNVNPQIIESYDGKDIVLNEQLQVTLSPDQFPELSGYKGHTLITTDGTTLLGADNKAGIAEIMTAMDYLIKHPEIKHGTIRVAFTPDEEIG-R-GPHKFDVKRFNASFAYTVDGGPLGELEYESFNAAAAKITIKGNNVHPGTAKGKMINSAKIAMKLNSLLPADEAPE-Y-----TEGYEGFYHLLSIQ--GDVEETKLHYIIRDFDKENFQNRKETMKRAVEELQNEYGQDRILLDMNDQYYNMREKIEPVIEIVNIAKQAMENLGIEPKISPIRGGTDGSQLS-YMGLPTPNIFTGGE-NFHGKFEYISVDNMVKAVNVIVEIAKQFEAQA 4Q7A Chain:A ((12-360)) -SMTTADLLRGLVSIPSPSGAE----------APAVEWLCQQMAALGYQ-AEPDGAGNAVGTRGE----GPREIMLLGHIDTVPGEV----------PV---QVV----------------------DGVLYGRG----AVDAKGPLATFVVAGARAKL---PPGVRLTVVGAVEEEVMSSRGARHLIATREAPDAVVIGEPSGWDGVVLGYRGSVALEYRVTVPMSHSAGPE---ATAAELAADFWYRLRTWCAEWSVGIDHAFHRVEPKLNALNSSSDGLYGEAVARIGLRLPPALSPEEAIAVATSLASEG-------EVTAT--VNAPAFQT--DKRQPIVAAFLAAVRAHGGTPRLKLKTGTSDMNLVGPAWGCPIVAYGPGDSRLDHTPEEHVPLADLERATAILTTAIERVAAQ-

General information: TITO was launched using: RESULT: Template: 4Q7A.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1980 -11732 -5.93 -34.81 target 2D structure prediction score : 0.55 Monomeric hydrophicity matching model chain A : 0.67 3D Compatibility (PKB) : -5.93 2D Compatibility (Sec. Struct. Predict.) : 0.55 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.451

(partial model without unconserved sides chains): PDB file : Tito_4Q7A.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4Q7A-query.scw PDB file : Tito_Scwrl_4Q7A.pdb: