Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEHLPEQYRQLFPTL------QTHTMLASCSQSALAEPVSRAIQDYYDSLLY-KGT--NWK--EAIEKTEFARNEFAKLIGAE-PDEVAIVPSVSDALVSVASSLT---AFGKKHVVYTDMDFPAVPHVWQAHS---DYTVSVIPS-IDGVLPLEQYETHISDETVLTCVPHVHYRDGYVQDIKAIAEISQRKGSLLFVDAYQSAGHIPIDVKEWGVDMLAAGTRKYLLGIPGVAFLYVRKELADALKPKASAWFGRESGFD--GAYAKVARRFQTGTPAFISVYAAAAALSLLNHIGVSHIRDHVKTICADAVQYAAEK-GLQLAAAQG---GIQPGMVAIRDER--ASETAGLLKK-KKVICAPR------------ENVIRLAPHFYNTKEEMRHAIDEIAAKTIHK 4Q75 Chain:A ((19-424)) ---LGHRVRKDFRILHQEVNGSKLVYLDSAATSQKPAAVLDALQNYYEFYNSNVHRGIHYLSAKATDEFELARKKVARFINASDSREIVFTRNATEAINLVAYSWGLSNLKPGDEVILTVAEHHSCIVPWQIVSQKTGAVLKFVTLNEDEVPDINKLRELISPKTKLVAVHHVSNVLASSLPIEEIVVWAHDVGAKVLVDACQSVPHMVVDVQKLNADFLVASSHKMC-GPTGIGFLYGKSDLLHSMPPFLGGGEMISDVFLDHSTYAEPPSRFEAGTPAIGEAIALGAAVDYLSGIGMPKIHEYEVEIGKYLYEKLSSLPDVRIYGPRPSESVHRGALCSFNVEGLHPTDLATFLDQQHGVAIRSGHHAQPLHRYLGVNASARASLYFYNTKDDVDAFIVALADTVSF-

General information: TITO was launched using: RESULT: Template: 4Q75.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2127 -268265 -126.12 -734.97 target 2D structure prediction score : 0.68 Monomeric hydrophicity matching model chain A : 0.74 3D Compatibility (PKB) : -126.12 2D Compatibility (Sec. Struct. Predict.) : 0.68 1D Compatibility (Hydrophobicity) : 0.74 QMean score : 0.499

(partial model without unconserved sides chains): PDB file : Tito_4Q75.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4Q75-query.scw PDB file : Tito_Scwrl_4Q75.pdb: