Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ------------------------------------------------------------------------------------MTTENQNPLVLDKNEEISQQKADDIRLQ---------LRQEP-----EVKRLAQQIDVKNQMELLEYGKEP-------AVEISKFSDRILGMMKTTSV-TDSGTMLTQLGKIMDRFDKNDFDEP--KGLMAKIFKRGGSM--IEKIFKKYQTLGGEIEKIHVEISKYKDEMTK-----TNYTLDEMYENNIKYYMELEKYVVAGQMKLEEMQS-------ILPSYEEKAASGNQLAQMQLDTLRNGIQALEERVYDL-DMARMVALQTAPQIRLLQRGNAKLIGKINSAFIITIPIFKNGIIQAVTVKRQKLVADSMSELDRRTNEMLKRNAENISSQSVEIARM-AGRPSIDIETIESSW-----NTIVSGMQETKQIE-EENKRLRED---------GARRIAQLQDNIKKAALQQ--- 4XA2 Chain:A ((1-490)) MKIEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPAAAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYAAGKYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSAVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFLENYLLT-DEGLEAVNKDKPLGAVALKSYEEELAKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDAALAAAQTNAAAA--------------YQNYIAKSQVSTGLADITAGKTNAETKLAEGLTAALTDVEALGLQKSTNACSTITTSIGTNGASNITCTLKGTSQINSKKIEWIRDADNATNGTTGAWRCKTDVAENLRPKSCGASGS

General information: TITO was launched using: RESULT: Template: 4XA2.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1430 -27748 -19.40 -79.73 target 2D structure prediction score : 0.48 Monomeric hydrophicity matching model chain A : 0.62 3D Compatibility (PKB) : -19.40 2D Compatibility (Sec. Struct. Predict.) : 0.48 1D Compatibility (Hydrophobicity) : 0.62 QMean score : 0.201

(partial model without unconserved sides chains): PDB file : Tito_4XA2.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4XA2-query.scw PDB file : Tito_Scwrl_4XA2.pdb: