Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAENQEVYDVTIIGGGPIGLFTAFYCGMREL-KTKVIEFLPKLGGKVSLFFPEKIIRDIGGIP-GIAGKQLIEQLKEQAATFDPDIVLNQRVTGFERLDDGTIVLTGSEGKKHYTRTVILACGMGTLEVNEFDSEDAARYAGKNLH-YGVEKLDAFKGKRVVISGGGDTAVDWANELEPIAASVTVVHRREEFGGMESSVT-KMKQSSVRVLTPYRLEQLNGDEEGIK--SVTVCHTESGQRKDIEIDELIINHGFKIDLGPMMEW----GLEIE-EGRVKADRHMRTNLPGVFVAGDAAFYESKLRLIAGGFTEGPTAVNSAKAYLDPKAENMAMYSTHHKKLVHK 3R9U Chain:A ((2-314)) ----NAMLDVAIIGGGPAGLSAGLYATRGGLKNVVMFEKGM-PGGQITSSSE---IENYPGVAQVMDGISFMAPWSEQCMRFGLKHE-MVGVEQILKNSDGSFTIKLEGGKTELAKAVIVCTGS-A--PKKAGFKGEDEFFGKGVSTCATCDGFFYKNKEVAVLGGGDTALEEALYLANICSKIYLIHRRDEFRAAPSTVEKVKKNEKIELITSASVDEVYGDKMGVAGVKVKLKD---GSIRDLNVPGIFTFVGLNVRNEILKQDDSKFLCNMEEGGQVSVDLKMQTSVAGLFAAGDLRKD--APKQVICAAGDGAVAALSAMAYIESL-----------------

General information: TITO was launched using: RESULT: Template: 3R9U.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1766 -205449 -116.34 -680.29 target 2D structure prediction score : 0.47 Monomeric hydrophicity matching model chain A : 0.73 3D Compatibility (PKB) : -116.34 2D Compatibility (Sec. Struct. Predict.) : 0.47 1D Compatibility (Hydrophobicity) : 0.73 QMean score : 0.519

(partial model without unconserved sides chains): PDB file : Tito_3R9U.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3R9U-query.scw PDB file : Tito_Scwrl_3R9U.pdb: