Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSQHVETKLAQIGNRSDEVTGTVSAPIYLSTAY--------RHRGIGESTGFDYVRTKNPTRQLVEDAIANLENGARGLAFSSGMAAIQ-TIMALFKSGDELIVSSDLYGGTYRLFENEWKKYGLTFHYDDFSDEDCLRSKITPNTKAVFVETPTNPLMQEADIEHIARITKEHG--LLLIVDNTFYTPVLQRPLELGADIVIHSATKYLGGHNDLLAGLVVVKDERLGE-EMFQHQNAIGAVLPPFDSWLLMRGMKTLSLRMRQHQANAQELAAFLEEQEEISDVLYPG----------------KGGMLSFRLQKE-EWVNPFLKALKTICFAESLGGVESFITYPATQTHMDIPEEIRIANGVCNRLLRFSVGIEHAEDLKEDLKQALCQVKEGAVSFE 5DX5 Chain:B ((11-395)) ------TKAIHGGHIGDKQFGSLATPIYQTSTFIFDSAEQGGRRFAGEESGYIYSRLGNPTSTEVENKLALLEGGEAAVVAASGMGAIAASLWSALKSGDHVVASDTLYGCTFALLNHGLTRYGVEVTFVDVSNLDEVKNALKPNTKVVYLETPANPTLKVTDIRKISNMVHESNKECFVFVDNTFCTPYIQRPLELGADVVVHSATKYLNGHGDVIAGFAVGKEEFINQVKLFGIKDMTGSVTGPFESFLIIRGMKTLQLRMEKHCKNAMEVAKFLESHPAVEKVYYPGLESFKYYQLAREQMKLPGAMISFELKGGVEEGKIVMNNVKLATLAVSLGDSETLIQHPASMTHSPYTAEERKAAGISDGLVRLSVGLEDAEDIIDDLKQAL-----------

General information: TITO was launched using: RESULT: Template: 5DX5.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 2114 -198123 -93.72 -556.52 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain B : 0.81 3D Compatibility (PKB) : -93.72 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.81 QMean score : 0.528

(partial model without unconserved sides chains): PDB file : Tito_5DX5.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5DX5-query.scw PDB file : Tito_Scwrl_5DX5.pdb: