Modeling by threading (Tito software)
Unconserved sides chains calculation (Scwrl software)
Evaluation (QMean software)



Input alignment information:
Query sequenceMIPLHENLAGKTAVITGGSGVLCSAMARELARHGMKVAILNRTAEKGQAVVKEITAAGGTACAVAADVLDRMSLERAKEDILGQFGAVDLLINGAGGNHPDAITDVETYEEAGEGQSFFDMDERGFLTVFSTNFTGAFLASQVFGKELLKADSPAIINLSSMSAYSPMTKVPAYSAAKASINNFTMWMAVHFAETGLRVNAIAPGFFLTKQNHDLLINQDGTFTSRS-HKIIAGTPMKRFGKPEDLLGTLLWLADESYSGFVTGITVPVDGGFMAYSGV
4NBW Chain:D ((6-257))----------RVAIITGAANGIGRATALEFAQAGYHVVAWDLAEEAGAALIAEIADAGGSADFARVDVSAAESVEAAVAEIIAEHGRVDVLVNNAGILHDGQLVKVKG------GEVVKKMAEAQFDAVISVNLKGVFLCTQAVAPHMIAAKYGRILNASSVVGLYGNFGQTNYVAAKSGVIGMTKVWARELGRYGITVNAIAPGFIATE-----MVQQ---MPERVLEAMVARTPVGRIGDPVDIARAYLFLASEE-SGFISGTTLSVDGGMVVGS--


General information:
TITO was launched using:
RESULT:

Template: 4NBW.pdb
Alignment : align.pir
Tito was launched with SMD and SCWRL
Tito text output
Monomeric PKB - chain D - contact count / total energy / energy per contact / energy per residue : 1353 -159847 -118.14 -636.84
target 2D structure prediction score : 0.59
Monomeric hydrophicity matching model chain D : 0.79

3D Compatibility (PKB) : -118.14
2D Compatibility (Sec. Struct. Predict.) : 0.59
1D Compatibility (Hydrophobicity) : 0.79
QMean score : 0.559

(partial model without unconserved sides chains):
PDB file : Tito_4NBW.pdb:





(Unconserved sides chains are recalculated) :
Sequence: align-4NBW-query.scw
PDB file : Tito_Scwrl_4NBW.pdb: