Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLEVKLPGLDLKNPIIPASGCFGFGKEFSRFYDLSCLGAIMIKATTKEPRFGNPTPRVAETGAGMLNAIGLQNPGLDSVLHHELPWLEQFDTPIIANVAGSQVDDYVEVAEHISKA-P-NVHALELNISCPNVKTGGIAFGTNPEMAADLTKAVKEVSDVPVYVKLSPNV--ANITEIALAIEE-AGADGLTMINTLI-GMRLDLKTGKPIL--ANKTGGLSGPAVKPVAIRMVYEVSQMV-NIPIIGMGGVQTAEDALEFLLAGASAVAVGTANFV-NPFACPEIIEQLPSVLLQYGYQSIEECIGRSWNHEKQPAHHRA 2E6F Chain:A ((3-313)) CLKLNLLDHVFANPFMNAAGVLCSTEEDLRCMTASSSGALVSKSCTSAPRDGNPEPRYMAFPLGSINSMGLPNLGFDFYLKYASDLHDYSKKPLFLSISGLSVEENVAMVRRLAPVAQEKGVLLELNLSCPNVPG-KPQVAYDFEAMRTYLQQVSLAYGLPFGVKMPPYFDIAHFDTAAAVLNEFPLVKFVTCVNSVGNGLVIDAESESVVIKPKQGFGGLGGKYILPTALANVNAFYRRCPDKLVFGCGGVYSGEDAFLHILAGASMVQVGTALQEEGPGIFTRLEDELLEIMARKGYRTLEEFRGRVKTI---------

General information: TITO was launched using: RESULT: Template: 2E6F.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1814 -197861 -109.07 -657.35 target 2D structure prediction score : 0.70 Monomeric hydrophicity matching model chain A : 0.75 3D Compatibility (PKB) : -109.07 2D Compatibility (Sec. Struct. Predict.) : 0.70 1D Compatibility (Hydrophobicity) : 0.75 QMean score : 0.471

(partial model without unconserved sides chains): PDB file : Tito_2E6F.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2E6F-query.scw PDB file : Tito_Scwrl_2E6F.pdb: