Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSLAPHGGT-LVNRVDESYD-V-------SGIQKEIELDLISFADLELIGIGAYSPIEGFFNEKDYVSVVENMRLSSGVVWSLPITLPVDAQKAAELSLGETVKLTY---EGE-TYGVIQIEDLYVP---DKQKEAVNVYKTDEQEHPGVKKLFSRGNTYVGGPITLIKKA-S-KQFPEFTFEPSETRRQFAEKGWETIVGFQTRNPVHRAHEYIQKTALET--VDGLFLNPLVGETKSDDIPADVRMESYQVLLDHYYPKDRVFLGVFLAAMRYAGPREAIFHALVRKNYGCTHFIVGRDHAGVGDYYGTYEAQELFDTFK-PEELGITPLKFEHSFFCKKCGNMGTAKTCP-HGREHHVILSGTKVRGMLRDGVLPPAEFSRKEVVEVLIKGMKKKEEVGVS 4DNX Chain:A ((1-395)) -MIKPVGSDELRPRFVYDPEQHHRLSSEAESL-PSVIVSSQAAGNAVMLGAGYFSPLDGFMNLADALSSAQSMTLTDGRFFPVPLLCLLESA--DAIAGATRIALRDPNVEGNPVLAVMDVTAVEQVSDAQMALMTEQVYGTSDPKHPGVETFNSQGRTAISGPIQVLNFSYFQTDFPDTFRTAVEIRHEIQERGWQKIVAFQTRNPMHRAHEELCKMAMEAVEADGVVIHMLLGQLKPGDIPAPVRDAAIRTMAELYFPPNTVMVTGYGFDMLYAGPREAVLHAYFRQNMGATHFIIGRDHAGVGDYYGPFDAQTIFDDAVPTDVLAIEIFRADNTAYSKKLGRVVMMRDAPDHTPDDFIQLSGTRVREMLGQGEAPPPEFSRPEVAQILMDYYRSLP-----

General information: TITO was launched using: RESULT: Template: 4DNX.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2043 -229963 -112.56 -616.52 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain A : 0.78 3D Compatibility (PKB) : -112.56 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.78 QMean score : 0.512

(partial model without unconserved sides chains): PDB file : Tito_4DNX.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4DNX-query.scw PDB file : Tito_Scwrl_4DNX.pdb: