Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ----------------MNLIGYIEE----RFQNLELIPSIYNQWGT-------GIHFCLGENIYQLKANEELNLKM---FRIVYEQTSIIF-----NELFE----------QNDDIFLVTNMYKHKKKEKCIRKLK--VYQP----FLKCKNH-LNQIMVKTYPYPF-EINKAEEYEMQQFSLLC------------KPRDLRVTELL--------KAASNEDFPQKPK-FGGYSIDYPD-VFFV---NITKDIIFFIYDDRGCEVIAH---DFKRIRPLYEKYHD--WVE-----EYKCM---- 4OVY Chain:A ((1-305)) EEPLPSWNDSAARRAILEYVKSVTTEGSPRFVPVSERIVTFDNDGTLWCEQPMYVQLAFALDRVRLLADKHPEWRTEEPFRAVIEKDLPALAKLGAKGLTELTMATHAGMTDDEFENIVTEWIRKARHPKFHRPYTECVYQPMLELLAFLRQHEFKTFIVSGAGIEFMRPWAKEVYGIPPEQVIGSSVKLKYELRDGKPVLVRLAELNFIDDQAGKPVGIRQVIGRRPVMAVGNSDGDYEMLEYVTSGPANGLGLIVHHTDAVREFAYDRQSPFGRLDRALTDATSKGWIVIDMQRDWKVIFPES

General information: TITO was launched using: RESULT: Template: 4OVY.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 845 -77413 -91.61 -363.44 target 2D structure prediction score : 0.30 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : -91.61 2D Compatibility (Sec. Struct. Predict.) : 0.30 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.231

(partial model without unconserved sides chains): PDB file : Tito_4OVY.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4OVY-query.scw PDB file : Tito_Scwrl_4OVY.pdb: