Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKFIKFNDSTIDSFLFMMLTDLAKTLTKSEAVEVEYGVQSYYNPFEKKIYMSHFWKDRAAEDMEAGLKSDVYLRSVGTRYSSLHEFANFLNDIHRHLTFKSFAKQLFMLLEDIRIEECIKRERPGTKHVFAKRKDMYRKHFSTQLTLNLERSIFTDALFCAIYFKLTAESPLETLPSMREDIDLMRPFIEQQLLRVYEADSTRQVLKIVEDLMDGLEEVLDKDMLNTYFFLPELDYAKAAEQPLFEEEKKAPKLSDDITLPKQSDGDEDIHEEEMPTWHRETEAPSKSFLQFDIEHGAKSDLGKDASREGDDGDQALGSVQGSARQTKRKDYSKLEALESQKDQPNGAGMADGKENKYAFPIYKEPQPATSEEELSYKQQAKTIESYQKRLKQMIQKTLEHKKTLPRTDLHAGRLNNKLLRYFTERNPRLFYKKQEPSSEIDAVFTLLVDCSASMFDKMDETKRGIVLFHEALKSV--AVPHQIVGFWEDTNDA-TEKS----------------QPNY-FNTVIPFQSSLRQDSGPAIMQLEPEEDN--R--DGYAIRQMTK---KMLHRSEAQKFLIVFSDGEPAAFG----------------------YEQNG---IVDTSEAVIEARKRGIEVINVFLSNSEIEESQMKTIQDMYGKFSI-FVP-DVDQLPDVLYPLLKKLLHKSIG 3T3P Chain:B ((96-351)) ------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------DSKNFSIQVRQVEDYPVDIYYLMDLSYSMKDDLWSIQNLGTKLATQMRKLTSNLRIGFGAFVDKPVSPYMYISPPEALENPCYDMKTTCLPMFGYKHVLTLTDQVT-RFNEEVKKQSVSRNRDAPEGGFDAIMQATVCDEKIGWRNDASHLLVFTTDAKTHIALDGRLAGIVQPNDGQCHVGSDNHYSASTTMDYPSLGLMTEKLSQKNINLIFAVTENVV---NLYQNYSELIPGTTVGVLSMDSSNVLQLIVDAYGKI------

General information: TITO was launched using: RESULT: Template: 3T3P.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 960 -4520 -4.71 -22.38 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain B : 0.60 3D Compatibility (PKB) : -4.71 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.60 QMean score : 0.464

(partial model without unconserved sides chains): PDB file : Tito_3T3P.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3T3P-query.scw PDB file : Tito_Scwrl_3T3P.pdb: