Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MFEWNKYFPFHNQFSKEALKKADPKEVETYVNRVMESVFGSDYAAQFPFRDPLPQKEHPAKPDAKPDVKPDIDIFETADHVFVKVPISEEWLEQVRIKHTSHELWLENLPRADHPKKVNLPCLVKRKGTKAVYKDGLLEVMFQKQQ---DYNMSEVEIIR 4W78 Chain:B ((1-126)) --------------MTVVGAVLPELKLYGDPTFIVSTALAT--------RDF--QDVHHDRDKAVAQGSKDIFVNILTDTGLVQRYVTDWAGPSALIKSIGLRLGVPWYAY----DTVTFSGEVTAVN------DGLITVKVVGRNTLGDHVTATVELSM

General information: TITO was launched using: RESULT: Template: 4W78.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 551 -28169 -51.12 -229.02 target 2D structure prediction score : 0.37 Monomeric hydrophicity matching model chain B : 0.60 3D Compatibility (PKB) : -51.12 2D Compatibility (Sec. Struct. Predict.) : 0.37 1D Compatibility (Hydrophobicity) : 0.60 QMean score : 0.110

(partial model without unconserved sides chains): PDB file : Tito_4W78.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4W78-query.scw PDB file : Tito_Scwrl_4W78.pdb: