Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKKLLMLTTGGTIASVEGENGLAPG-VKADELLSYVSKLDN--DYTMETQSLM-NIDSTNMQPEYWVEIAEAVKENYDAYDGFVITHGTDTMAYTSAALSYMLQHAKKPIVITGSQIPITFQKTDAKKNITDAIRFACEG-VGGVYVVFDGRVIQGTRAIKLRTKSYDAFESINYPYIAFINEDGIEYNKQVTEPE-NDTFTVDTSLCTDVCLLKLHPGLKPEMFDALKSM-YKGIVIESYGSGGVPFEGRDILSKVNELIESGIVVVITTQCLEEGEDMSIYEVGRRVNQDLIIRSRNMNTEAIVPKLMWALGQSSDLPVVKRIMETPIADDVVL 2HIM Chain:A ((23-356)) KKSIYVAYTGGTIGMQR-----IPVSGHLQRQLALMPEFHRPEMPDFTIHEYTPLMDSSDMTPEDWQHIAEDIKAHYDDYDGFVILHGTDTMAYTASALSFMLENLGKPVIVTGSQIPLAELRSDGQINLLNALYVAANYPINEVTLFFNNRLYRGNRTAKAHADGFDAFASPNLPPLLEAG-IHIRRLNTPPAPHGEGELIVHPITPQPIGVVTIYPGISADVVRNFLRQPVKALILRSYGVGNAPQNK-AFLQELQEASDRGIVVVNLTQCMSGKVNMG-----NALAHAGVIGGADMTVEATLTKLHYLLSQELDTETIRKAMSQNLRGELTP

General information: TITO was launched using: RESULT: Template: 2HIM.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1823 -8302 -4.55 -26.19 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain A : 0.78 3D Compatibility (PKB) : -4.55 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.78 QMean score : 0.570

(partial model without unconserved sides chains): PDB file : Tito_2HIM.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2HIM-query.scw PDB file : Tito_Scwrl_2HIM.pdb: