Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MMKATIVLDAKGLACPMPIVKTKKRMKDLKAGEVLEIHATDKGSTADLEAWAKSTGHEYLGTEAEGEILRHFLRKGGEHSSENASSIPEISLEAFKQKVDSDESLNILDVREIEEYEKAHIPGVVHIPLGEVEKRANE----------------LNENDEIYIICHSGRRSEMAARTMKKQGF------KKVINVVPGMRDWTGKTE 3I2V Chain:A ((1-119)) ----------------------------------------------------------------------------------------RVSVTDYKRLLDSGAFHLLLDVRPQVEVDICRLPHALHIPLKHLERRDAESLKLLKEAIWEEKQGT--AAVPIYVICKLGNDSQKAVKILQSLSAAQELDPLTVRDVVGGLMAWAAKI-

General information: TITO was launched using: RESULT: Template: 3I2V.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 296 -34230 -115.64 -364.15 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : -115.64 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.586

(partial model without unconserved sides chains): PDB file : Tito_3I2V.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3I2V-query.scw PDB file : Tito_Scwrl_3I2V.pdb: