Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAKQLIQSEEEFKRIAEQEGVFVFLKHSTTCPISQAAFHEFDAFANQHEDVPAYYLQVQEARPLSNFIAETYGVKHESPQIFIIQNGEVKWHTSHSQITEAAIEQHLS 2E0Q Chain:A ((41-134)) -------DSKNFDSFLASHEIAVVDFWAEWCAPCLILAPIIEELAEDYPQVGFGKLNSDENPD----IAARYGVMS-LPTVIFFKDGEPVDEII-GAVPREEIEIRI-

General information: TITO was launched using: RESULT: Template: 2E0Q.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 378 -61106 -161.66 -650.06 target 2D structure prediction score : 0.70 Monomeric hydrophicity matching model chain A : 0.68 3D Compatibility (PKB) : -161.66 2D Compatibility (Sec. Struct. Predict.) : 0.70 1D Compatibility (Hydrophobicity) : 0.68 QMean score : 0.761

(partial model without unconserved sides chains): PDB file : Tito_2E0Q.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2E0Q-query.scw PDB file : Tito_Scwrl_2E0Q.pdb: