Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ---------------------------------------------------------------------------------------------------------------------MKTKVVMCSGLFCSVFAGAFMLNQYDGRSGVAACDEWELYLLEH-HLSARMSETESKDLPFGPREYIRIVNK----------------------------- 1BIF Chain:? ((38-250)) CPTLIVMVGLPARGKTYISKKLTRYLNFIGVPTREFNVGQYRRDMVKTYKSFEFFLPDNEEGLKIRKQCALAALNDVRKFLSEEGGHVAVFDATNTTRERRAMIFNFGEQNGYKTFFVESICVDPEVIAANIVQVKLGSPDYVNRDSDEATEDFMRRIECYENSYESLDEEQDRDLS-----YIKIMDVGQSYVVNRVADHIQSRIVYYLMNIHVTPR

General information: TITO was launched using: RESULT: Template: 1BIF.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 112 7579 67.67 114.83 target 2D structure prediction score : 0.48 Monomeric hydrophicity matching model chain A : 0.56 3D Compatibility (PKB) : 67.67 2D Compatibility (Sec. Struct. Predict.) : 0.48 1D Compatibility (Hydrophobicity) : 0.56 QMean score : 0.129

(partial model without unconserved sides chains): PDB file : Tito_1BIF.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1BIF-query.scw PDB file : Tito_Scwrl_1BIF.pdb: