Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNVLNKIALVTGGGTGIGKAASMELAKRGAIVAVNYSRSQSEAEETVEMIQKAGGQAFAIQADVSKNSDVQDMIQAIVNTHGTVDILVNNASITRHIPMDDLEAATEDV 1RWB Chain:B ((8-95)) -----KVVVITGSSTGLGKSMAIRFATEKAKVVVNYRSKEDEANSVLEEIKKVGGEAIAVKGDVTVESDVINLVQSAIKEFGKLDVMINNAGL----------------

General information: TITO was launched using: RESULT: Template: 1RWB.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 430 -39352 -91.52 -447.18 target 2D structure prediction score : 0.76 Monomeric hydrophicity matching model chain B : 0.80 3D Compatibility (PKB) : -91.52 2D Compatibility (Sec. Struct. Predict.) : 0.76 1D Compatibility (Hydrophobicity) : 0.80 QMean score : 0.740

(partial model without unconserved sides chains): PDB file : Tito_1RWB.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1RWB-query.scw PDB file : Tito_Scwrl_1RWB.pdb: