Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTHIYKKLGAAFFALLLIAALAACGNNSESKGSASDSKGAETFTYKAENGNVKIPKHPKRVVVMADGYYGYFKTLGINVVGAP----ENVFKNPYYKGKTNGVENIGD--GTSVEKVIDLNPDLIIVWTTQG----ADIKKLEKIAPTVAVKYDKLDNIEQLKEFAKMTGTEDKAEKWLAKWDKKVAAAKTKIKKAVGDKTISIMQTNGKDIYVFGKDFGRGGSIIYKDLGLQATKLTKEKAIDQGPG--YTSISLEKLPDFAGDYIFAGPWQSGGDDGGVFES-SIWKNLNAVKNGHVYKMDPIGFYFTDPISLEGQLEFITESLTK---------- 3R5T Chain:A ((3-298)) ------------------------------------NVWPRTFQN-ADGSITTIPSQPKRILSTAVTVTGTLLAIDAPVIASAATTQSTFFEQWRKLAELRQVKKLWPAGSVDLESVYVEQPDLIVVSMIGADSARDQIPLLQAIAPTILVDYSDQTWQSLAQQLGLATGLEEQAERTIHNFEQWTKQVRDVLDLPKGRANIVSYHGPGVVNAVAKAQ--SAHAQLLQSVGVVLEEPDPAWQAGSIVHRDFLRIHYEHLTQLQAETTFL--ITMTDQQAQAFLHDPILKNLPSIQRKQVYGLGE-NSFRIDLFSAREIINSLLRRFAGEQAQSLVMPL

General information: TITO was launched using: RESULT: Template: 3R5T.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1500 29710 19.81 108.83 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain A : 0.67 3D Compatibility (PKB) : 19.81 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.586

(partial model without unconserved sides chains): PDB file : Tito_3R5T.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3R5T-query.scw PDB file : Tito_Scwrl_3R5T.pdb: