Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MQLQVMNSPFNQEQAELLNRLLPTLTESQKIWLSGYLSAQSVSAQEAAGTPAAAVSAEAPAPAVSKEVTVLYGSQTGNAQGLAENAGKQLEQSGFQVTVSSMSDFKPNQLKKVTNLLIVVSTHGEGEPPDNALSFHEFLHGRRAPKLEDLRFSVLALGDSSYEFFCQTGKEFDQRLEELGGKRISPRVDCDLDYDEPAAEWLEGVLKGLNEAGGGSAAPAPAAASQTGESSYSRTNPFRAEVLENLNLNGRGSNKETRHVELSLEGSGLTYEPGDSLGVYPENDPELVELLLKEMNWDPEEIVTLNKQGDVRPLK-----EALISHYEIT--VLTKPLLEQAAQLTGNDELRELLAPGNEENVKAYIEGRDL--LDLVRDYGPFSVSAQEFVSILRKMPARLYSIASSLSANPDEVHLTIGAVR---YDAHGRERKGVCSILCAERLQPGDTLPVYVQHNQN-FKLPKDPETPIIMVGPGTGVAPFRSFMQEREETGAEGK----AWMFFGDQHFVTDFLYQTEWQNWLKDGVLTKMDVAFSR-DTEEKVYVQHRMLEHSAELFEWLQEGAAVYICGDEKHMAHDVHNTLLEIIEKEGNMSREEAEAYLADMQQQKRYQRDVY 4DQL Chain:A ((7-391)) ---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------FSTNVVASKELQQPGSARSTRHLEIELPKEA-SYQEGDHLGVIPRNYEGIVNRVTARFGLDASQQIRLEA--------KTVSVEELLQYVELQDPVTRTQLRAMAAKTVCPPHKVELEALLEKQAYKEQVLAKRLTMLELLEKYPACEMKFSEFIALLPSIRPRYYSISSSPRVDEKQASITVSVVSGEAWSGYG-EYKGIASNYLAE-LQEGDTITCFISTPQSEFTLPKDPETPLIMVGPGTGVAPFRGFVQARKQLKEQGQSLGEAHLYFGCRSPHEDYLYQEELENAQSEGIIT-LHTAFSRMPNQPKTYVQHVMEQDGKKLIELLDQGAHFYICGDGSQMAPAVEATLMKSYADVHQVSEADARLWLQQLEEKGRYAKDVW

General information: TITO was launched using: RESULT: Template: 4DQL.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1757 77346 44.02 217.26 target 2D structure prediction score : 0.68 Monomeric hydrophicity matching model chain A : 0.69 3D Compatibility (PKB) : 44.02 2D Compatibility (Sec. Struct. Predict.) : 0.68 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.563

(partial model without unconserved sides chains): PDB file : Tito_4DQL.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4DQL-query.scw PDB file : Tito_Scwrl_4DQL.pdb: