Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTAHDQELRRRAYEEVEKKEPIANSDPHRQHFHIMPPVGLLNDPNGVIYWKGSYHVFFQWQPFQTGHG-AKFWGHYTTQDVVNWKREEIALAPSDWFDKNGCYSGSAVTK-DDRLYLFYTGNVRDQDGNRETYQCLAVSDD-----GLSFEKKG--VVARLPEG-YTAHFRDPKVWEH--EGTWYMVIGAQTENLKGQAVLFASDNLTEWRFL-GPITGAGFNGLDDFGYMWECPDLFSLQG---------------SDVLIVSPQGLEADGFRYQNVYQSGYFVGRLDYNKPELKHGE---------FTELDQGFDFYAPQTLEDD-QGRRILFAWMAVPDQDEGSHPTIDCHWIHCMTLPRQLTLS-GQ-KLIQQPLPELKAMRRNEKKI-HINMHGSSGALPV--EKPERTEILLE-DIH----------------------------TESGFSISIRGT------ATFSFHKDEGIV-------TLERKSFDG--K-----RTEARHCRI-KDLHTVHMFLDASSVEIFINNGEEVLSARYFPFPGNH---EVTASATGK---SEMNVGIWTLM 1ST8 Chain:A ((3-529)) -----------------------IEQPYRTGYHFQPPSNWMNDPNGPMLYQGVYHFFYQYNPYAATFGDVIIWGHAVSYDLVNWIHLDPAIYPTQEADSKSCWSGSATILPGNIPAMLYTGSDSK----SRQVQDLAWPKNLSDPFLREWVKHPKNPLITPPEGVKDDCFRDPSTAWLGPDGVWRIVVGGDRD-NNGMAFLYQSTDFVNWKRYDQPLSSA------DATGTWECPDFYPVPLNSTNGLDTSVYGGSVRHVMKAGFE------------GHDWYTIGTYSPDRENFLPQNGLSLTGSTLDLRYDYG-QFYASKSFFDDAKNRRVLWAWVPETDSQ---ADDIEKGWAGLQSFPRALWIDRNGKQLIQWPVEEIEELRQNQVNLQNKNLKP-GSVLEIHGIAASQADVTISFKLEGLKEAEVLDTTLVDPQALCNERGASSRGALGPFGLLAMASKDLKEQSAIFFRVFQNQLGRYSVLMCSDLSRSTVRSNIDTTSYGAFVDIDPRSEEISLRNLIDHSIIESFGAGGKTCITSRIYPKFVNNEEAHLFVFNNGTQNVKISEMSAWSMK

General information: TITO was launched using: RESULT: Template: 1ST8.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2285 -6781 -2.97 -15.84 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain A : 0.71 3D Compatibility (PKB) : -2.97 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.71 QMean score : 0.434

(partial model without unconserved sides chains): PDB file : Tito_1ST8.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1ST8-query.scw PDB file : Tito_Scwrl_1ST8.pdb: