Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKRRVETITFDGGTLEYSVTGK--GTPILVMHG--GHSNCYEEFGYTALIEQGYSIITPSRPGYGRTSK---EIGKSLANACRFYVKLLDHLQIESVHVIAISAGGPSGICFASHY-PERVNTLTL---------QSAVTKEWLTPKDTEYKLGEILFRPPVEKWIWKLISSLNNAFPRLMFRAMSPQF-----------STLPFQRIKSLMNEKDIEAFRKMNSRQRSGEGFLIDLSQTAAVSLKDLQAIICPVLIMQSVYDGLVDLSH-AHHAKEHIRGAVLCLLHSWGHLIWL-GKEAAETGSILLGFLES 1A88 Chain:A ((1-275)) GTVTTSD----GTNIFYKDWGPRDGLPVVFHHGWPLSADD-WDNQMLFFLSHGYRVIAHDRRGHGRSDQPST--GHDMDTYAADVAALTEALDLRGAVHIGHSTGGGEVARYVARAEPGRVAKAVLVSAVPPVMVKS---DTNPDG-LPLEVFDEFR-------AALAA------NRAQFYIDVPSGPFYGFNREGATVSQGLIDHWWLQGMM-GAANAHYECIAAFSE-------TDF-TDDLK----RIDVPVLVAHGTDDQVVPYADAAPKSAELLANATLKSYEGLPHGMLST--HPEVLNPDLLAFVKS

General information: TITO was launched using: RESULT: Template: 1A88.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1291 -141142 -109.33 -576.09 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : -109.33 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.423

(partial model without unconserved sides chains): PDB file : Tito_1A88.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1A88-query.scw PDB file : Tito_Scwrl_1A88.pdb: