Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKALIYARVSTNKEQQETSLKRQEEELTAIAAEN-GMEVVKVISEK-ASGYEMDRDGVFELLDEIKNADIDVILVQDETRLGRGNAKI-ALLHCIYREGVKVYTTAHRGELELSEADSMVLEIVSIVEEYQRKIHNMKIRRGMKRAVKNGFKPQKNLKNQHGNSGKEKIEVPISEIVRLRANKLTFAEIAATLRGFGYDVSKATVHRRFQEYIENEETAE 3G13 Chain:A ((6-160)) LRVAAYCRVSTDSEDQLNSYKSQVQYYTDMIKKNKEWVLADIYADEA-----TKREDFQRMINDCMNGEIDMVFTKSISRFARNTLDTLKYVRMLKERNIAVYFEDEK-INTLTMDGELLLVVLSSVAQQEVENISANVKKGLKMKMKRGELVGF-----------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 3G13.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 534 3421 6.41 23.43 target 2D structure prediction score : 0.75 Monomeric hydrophicity matching model chain A : 0.68 3D Compatibility (PKB) : 6.41 2D Compatibility (Sec. Struct. Predict.) : 0.75 1D Compatibility (Hydrophobicity) : 0.68 QMean score : 0.528

(partial model without unconserved sides chains): PDB file : Tito_3G13.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3G13-query.scw PDB file : Tito_Scwrl_3G13.pdb: