Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MERISWNQYFMAQSHLLALRSTCPRLSVGATIVRDKRMIAG-GYNGSIAGGVHCADEGCLM-------IDDHCARTIHAEMNAILQCSKFGVPTDGAEIYVTHYPCIQCCKSIIQAGIKTVYYAEDYKTHPY-----AQELFEQAGVTVEQV--ELDEMIVDLKNREKLSFVAGLIGKLADAGLAEEELKKIHEQANTLFTSYV 2W4L Chain:E ((10-163)) ---LEWPEYFMAVAFLSAQRSKDPNSQVGACIVNSENKIVGIGYNGMPNG---CSDDVLPWRRTAENKLDTKYPYVCHAELNAIM--NKNLTDVKGCSMYVALFPCNECAKLIIQAGIKEVIFMSD-KYHDSDEATAARLLFNMAGVTFRKFIPKCSKIVIDF-----------------------------------------

General information: TITO was launched using: RESULT: Template: 2W4L.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain E - contact count / total energy / energy per contact / energy per residue : 663 -20177 -30.43 -145.16 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain E : 0.73 3D Compatibility (PKB) : -30.43 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.73 QMean score : 0.543

(partial model without unconserved sides chains): PDB file : Tito_2W4L.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2W4L-query.scw PDB file : Tito_Scwrl_2W4L.pdb: