Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MRLDKLLANSGYGSRKEVKAVVKAGAVMIDGKPAKDVKEHVDPDTQEVTVYGEPVDYREFIYLMMNKPQGVLSATEDSRQQTVVDLLTPEEMRFEPFPAGRLDKDTEGFLLLTNDGQLAHRLLSPKKHVPKTYEVHLKSQISREDISDLETGVYIEGGYKTKPAKAEIKTNDSGNTVIYLTITEGKYHQVKQMAKAVGNEVVYLKRLSMGRVSLDPALAPGEYRELTEEELHLLNEPQA 2GML Chain:A ((1-170)) ----------------------------------------------------------DLVLIALNKPVGIVSTTEDGERDNIVDFVNH---SKRVFPIGRLDKDSQGLIFLTNHGDLVNKILRAGNDHEKEYLVTVDKPITEEFIRGMSAGVPILG-TVTKKCKVK----KEAPFVFRITLVQGLNRQIRRMCEHFGYEVKKLERTRIMNVSLS-GIPLGEWRDLTDDELIDLFKL--

General information: TITO was launched using: RESULT: Template: 2GML.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 787 -72663 -92.33 -427.43 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain A : 0.74 3D Compatibility (PKB) : -92.33 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.74 QMean score : 0.487

(partial model without unconserved sides chains): PDB file : Tito_2GML.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2GML-query.scw PDB file : Tito_Scwrl_2GML.pdb: