Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKEEIIERFTTYVKVDTQSDESVDTCPSTPGQLTLGNMLVDELKSI---GMQDAAID----ENGYVMATLPSNTEKDVPTIGFLAHVDTATDFTGKNVNPQII--ESYDGKDIVLNEQLQVTLSPDQFPELSGYKGHTLITTDGTTLLGADNKAGIAEIMTAMDYLIKHPEIKHGTIRVAFTPDEEIGR-GPHKFDVKR---F-NASFAYTVDGGPLG----ELEYESFNAAAAKITIK--GNNVHPGTAKGKMINSAKIAMKLNSLLPADEA-----P--------------------------EYTE-------------------GYEGFYHLLSIQGD----------VEETKLHYIIRDFDKENFQNRKETMKRAVEELQNEYGQDRILLDMND-QYYNMREKIEPVIEIVNIAKQAMEN-LGIEPKISPIRGGTD-GSQLSY----M-GLPTPNIFTGGENFHGKFEYISVDNMVKAVNVIVEIAKQFEAQA 3PFE Chain:A ((18-471)) --EEILPSLCDYIKIPNKSPHFDAKWEEHGYMEQAVNHIANWCKSHAPKGMT-LEIVRLKNRTPLLFMEIPGQ---IDDTVLLYGHLDKQPEM--SGWS---DDLHPWKP-VLK----------------------NGLLYGRG----GADDGYSAYASLTAIRALEQQGL-PYPRCILIIEACEESGSYDLPFYIELLKERIGKPSLVICLDSGAGNYEQLWMTTSLRGNLVGKLTVELINEGVHSGSASGIVADSFRVARQLISRIEDENTGEIKLPQLYCDIPDERIKQAKQCAEILGEQVYSEFPWIDSAKPVIQDKQQLILNRTWRPALTVTGADGFPAIADAGNVMRPVTSLKLSMRLPPLVDPEAASVAMEKALTQNPP-YN-AKVDFKIQNGGSKGWNAP-LLSDWLAKAASEASMTYYDKPAAYMGEGGTIPFMSMLGEQFPKAQFMITGVLGPHS-NAHGPNEFLHLDMVKKLTSCVSYVLYSFSQK-

General information: TITO was launched using: RESULT: Template: 3PFE.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1673 43016 25.71 117.53 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : 25.71 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.419

(partial model without unconserved sides chains): PDB file : Tito_3PFE.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3PFE-query.scw PDB file : Tito_Scwrl_3PFE.pdb: