Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEKDGLCRADQQYECVAEIGEGAYGKVFKARDLKNGGRFVALKRVRVQTGEEGMPLSTIREVAVLRHLETFEHPNVVRLFDVCTVSRTDRETKLTLVFEHVDQDLTTYLDKVPEPGVPTETIKDMMFQLLRGLDFLHSHRVVHRDLKPQNILVTSSGQIKLADFGLARIYSFQMALTSVVVTLWYRAPEVLLQSS-YATPVDLWSVGCIFAEMFRRKPLFRGSSDVDQLGKILDVIGLPGEEDWPRDVALP--RQAFHSKSAQPIEKFVTDIDELGKDLLLKCLTFNPAKRISAYSALSHPYFQDLERCKENLDSHLPPSQNTSELNTA 4EK6 Chain:A ((5-292)) ----------ENFQKVEKIGEGTYGVVYKARN-KLTGEVVALKKI----------STAIREISLLKELN---HPNIVKLLDVIHT-----ENKLYLVFEFLHQDLKKFMDASALTGIPLPLIKSYLFQLLQGLAFCHSHRVLHRDLKPQNLLINTEGAIKLADFGLARA---------EVVTLWYRAPEILLGCKYYSTAVDIWSLGCIFAEMVTRRALFPGDSEIDQLFRIFRTLGTPDEVVWPGVTSMPDYKPSFPKWARQDFSKVVPPLDEDGRSLLSQMLHYDPNKRISAKAALAHPFFQDVTKPVPHLRL--------------

General information: TITO was launched using: RESULT: Template: 4EK6.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output 3D Compatibility (PKB) 10400 for 2077 contacts (5.0/contact) + 2D Compatibility (PS) -26579 + (NN) 316 + (LL) 2736 1D Compatibility (HY) 18000 + (ID) 950 Total energy: 3923.0 ( 1.89 by residue) QMean score : 0.153

(partial model without unconserved sides chains): PDB file : Tito_4EK6.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4EK6-query.scw PDB file : Tito_Scwrl_4EK6.pdb: