Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MILETTAKASQQYKVMPIMISLLLAGFIGMFSETALNIALTDLMKE-LNITAATVQWLTTGYLLVLGILVPVSGLLLQWFTTRQLFTV---SLIFSILGTFIAALAPS-FSFLLAARIVQALGTGL----LLPLMFNTILVIFPPHKRGAAMGTIGLVIMFAPAIGPTFSGLVLEHLNWHWIFWISLPFLVLALVFGIAYMQNVSETTKPKIDVLSIILSTIGFGGIVFGFSNAGEGSGGWSSPTVIVSLIVGVVGLILFSIRQLTMKQPMMNLRAFKYPMFILGVIMVFICMMVILSSMLLLPMYLQGG---LVLTAFASGLVLLPGGILNGFMSPVTGRLFDKYGPKWLVIPGFVIVTVVLWFFSNVTTTSTAVLIIILHTCLMIGISMIMMPAQTNGLNQLPREFYPDGTAIM-NTLQQMAGAIGTAVAVSIMAAGQHDYMSTVKNPADPAVIPQALTAGVQHAFVFAMIVAIIGLIGAFFMKRVKVDH 2CFQ Chain:A ((18-375)) ---------------------------FYFFIMGAYFPFFPIWLHDINHISKSDTGIIFAAISLFSLLFQPLFGLLSDKLGLRKYLLWIITGMLVMFAPFFIFIFGPLLQYNILVGSIVGGIYLGFCFNAGAPAVEAFIEKVS--RRSNFEFGRARMFGCVGWALGASIVGIMFT-INNQFVFWLGSGCALILAVLL-FFAKTDAPSSATVANAV------------------GAN---------------------------H-SAFSLKLALELFRQPKLWFLSLYVIGVSCTYDVFDQQFANFFTSFFATGEQGTRVFGYVTTMGELLNASIMFFAPLIINRIGGKNALLLAGTIMSVRIIGSSFA---TSALEVVILKTLHMFEVPFLLVGCFKYITSQFEVRFSATIYLVCFCFFKQLAMIFMSVLAGNMYES------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 2CFQ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1484 -262826 -177.11 -761.81 target 2D structure prediction score : 0.50 Monomeric hydrophicity matching model chain A : 0.62 3D Compatibility (PKB) : -177.11 2D Compatibility (Sec. Struct. Predict.) : 0.50 1D Compatibility (Hydrophobicity) : 0.62 QMean score : 0.163

(partial model without unconserved sides chains): PDB file : Tito_2CFQ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2CFQ-query.scw PDB file : Tito_Scwrl_2CFQ.pdb: