Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKGAAFVKKEGLKQKALEIGRVPTHLKLEIEDYGGDDKRAHFCWADPQDENTGIIVELGPDGELE-SLSRDIEPESGERLSEEKLEDIMRQFVETHHPGALSAFVREENDRAYGDKVRFSYVQMEAGLPLPMSGFMADVSLSGEIVYFRYYGEAGSIIKPK-RVADVEEALAFIKKDVEFDLLFEVLHRSVYKNGDDQPHLVYEPEGRAITVPADLVQEEQAVD-----------DDDDYREPESFPLPLFEGIREKADPDSMIGIENGFVKEREADLGDGRIGIVWRNPDDPVYQPADKSMDSWFKGRTHQVLKTIYNKETGKLEGVMSFMEKKGPLTVTLAECEKIALRFLFALFPNADQYFRIRYDEKDEEENAVA---GFTFEAHCHGVPLRFGQIRICVSRQTGY-----------ITVYMGPDIDPNKLATIDPVPAISVEQAKSIFWQHFKVELGWEREYGDDEEHSYRLVYKPVYPHFIDAHTGEPVFSIW 4ZKD Chain:A ((7-499)) IDIHSFIATHPLNLTCLFLGD---------TNAGKSTLLGHLLYDLNEISMSSMRELQKKSSNLDPSSSNSFKVILDNTKTERENGFSMFKKVIQVENDLLPPSSTLTLIDTPGSIKYFNKETLNSILTF-------DPEVYVLVIDCNYDSWEKSLDGPNNQIYEILKVISYLNKNSACKKHLIILLNKADLISWDKHRLEMIQSELNYVLKENFQWTDAEFQFIPCSGLLGSNLNKINYVPEWYEGPTFFSQLYLLVEHNMNKIET---TLEEPFVGTILQSSVLQPIAEINYVSLKVLINSGYIQ-SGQTIEIHTQYEDFHYYGIVSRMKNSKQILETNTKN-----NISVGLNPDILEVLVKIHNTEDFTKKQFHIRKGDIIIHSRKTNTLKLLALRLIKLSIQTHALSDPVDLGSELLLYHNLTHNAVKLVKILGTNDISINPNQSLIVEVEIIEPDFALNVIDSKYITNNIVLTSI-DHKVIAVGRIACQ---

General information: TITO was launched using: RESULT: Template: 4ZKD.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2264 -99873 -44.11 -225.45 target 2D structure prediction score : 0.38 Monomeric hydrophicity matching model chain A : 0.60 3D Compatibility (PKB) : -44.11 2D Compatibility (Sec. Struct. Predict.) : 0.38 1D Compatibility (Hydrophobicity) : 0.60 QMean score : 0.028

(partial model without unconserved sides chains): PDB file : Tito_4ZKD.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4ZKD-query.scw PDB file : Tito_Scwrl_4ZKD.pdb: