Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MQKLSEEMKQTIMDIFEHLHANPEVSWKEYETTSFLKQKLEDLGCRTRTFSDCTGVVGEIGS--GSPVVAVRADIDALWQEVN-------GTFRANHSCGHDSHMTMALGTLMLLKKQPELPKGTIRFIFQPAEEKGG-GALKMIEEGVLDDIDYLYGVHVRPIQETQNGRC--APSILHGSSQHIEGTIIGEEAHGARPHLGKNSIEIAAFLVHKLGLIH---IDPQIPHTVKMTKLQAGGESSNIIPGKASFSLDLRAQTNEAMEALIAETERACEAAAAAFGAKIELHKEHSLPAATQNKEAEAIMAEAITEIIGAERLDDPLVTTGGEDFHFYAVKVPNLKTTMLGLGCG--LQPGLHHPHMTFDRNAMFTGIHILANAVLKTFQKAESLAAANAS 4EWT Chain:A ((6-389)) -IETLKSKEGKMIEIRRYLHQHPELSFHEDETAKYIAEFYKGKDVEVETNVGPRGIKVTIDSGKPGKTLAIRADFDALPITEDTGLSFASQNKGVMHACGHDAHTAYMLVLAETLAEMKDSFTGKVVVIHQPAEEVPPGGAKTMIENGVLDGVDHVLGVHVMS--TMKTGKVYYRPGYVQTGRAFFKLKVQGKGGHGSSPHMANDAIVAGSYFVTALQTVVSRRLSPFETGVVTIGSFDGKG-QFNVIKDVVEIEGDVRGLTDATKATIEKEIKRLSKGLEDMYGVTCTLEYNDDYPALYNDPEFTEYVAKTLKEANLDFGVEMCEPQPPSEDFAYYAKERPS-AFIYTGAAVENGEIYPHHHPKFNISEKSLLISAEAVGTVVLDYLK-----------

General information: TITO was launched using: RESULT: Template: 4EWT.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2129 -154241 -72.45 -420.27 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain A : 0.71 3D Compatibility (PKB) : -72.45 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.71 QMean score : 0.491

(partial model without unconserved sides chains): PDB file : Tito_4EWT.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4EWT-query.scw PDB file : Tito_Scwrl_4EWT.pdb: