Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTNFILNENKQLSLAIEDENIENFYIDGTDLVRKIIRRSGSGVTSRVPVLSTQDLENKNLHELYDESWLRMKNRPNTELTTESINIADLFSGCGGLSLGVWEACRALGINP--RFSFACDLNEAALSVYEKNFSPDFSLNESIEKHINGELGAPLTVEEQRIKDKVKKIDFILAGPPCQGHSDLNNHTRRKDPRNALLMRVSRVIELFQ-PSSVLVENVPGIIHDKSGSFKEFKNHLKTQGYYFDEIVLNAEKLGVSQARRRYFIFASKTPVSSLNQINEFY--------STNSRPISWAISDLVENVGDDIFNTASEHSLENKRRIEYLFENNLFELPNSERPDCHRLKPHSYKSVYGRMYWDRPAPTITRGFGSTG-----QG---RFVHSLLKRTITPHEAARIQFFPDFFNFGDLR--RRQYQDVIGNAVPSKLSYLLALHQLR 4H0N Chain:A ((3-333)) ----------------------------------------------------------------------------------SHKILELYSGIGGMHCAWKES------GLDGEIVAAVDINTVANSVYKHNFPETNLLNRNIQQLTPQVI-------------KKWNVDTILMSPPCQPFTRNGKYLDDNDPRTNSFLYLIGILDQLDNVDYILMENVKGFENST--VRNLFIDKLKECNFIYQEFLLCPSTVGVPNSRLRYYCTARRNNLT-WPFKRRDEIITRLPKDFGVPHSLESIIEEDVDEKFL---------VPEKMLRCAKVFD--ICY-KTSKRSCCFTKAYTHYADGTGSIFTDKPREVVQKCYAAAAQNEIGGEKFVELFKELKLRYFTPKEVLMIMCFPKSYNLPTNISMKQCYRL-LGNSVNVKVISELLKILFE

General information: TITO was launched using: RESULT: Template: 4H0N.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1563 -161178 -103.12 -519.93 target 2D structure prediction score : 0.49 Monomeric hydrophicity matching model chain A : 0.67 3D Compatibility (PKB) : -103.12 2D Compatibility (Sec. Struct. Predict.) : 0.49 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.368

(partial model without unconserved sides chains): PDB file : Tito_4H0N.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4H0N-query.scw PDB file : Tito_Scwrl_4H0N.pdb: