Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTIKRALISVSDKTNLVPFVKELTELGVEVISTGGTKKLLQENGVDVIGISEVTGFPEIMDGRLKTLHPNIHGGLLAVRGNEEHMAQINEHGIQPIDLVVVNLYPFKETISKEDVTYEEAIENIDIGGPGMLRAASKNHQDVTVIVDPADYSPVLNQIKEEGSVSLQKKRELAAKVFRHTAAYDALIADYLTNVVGEKEPEQFTVTFEKKQSLRYGENPHQEATFYQTALPVKGSIAQAEQLHGKELSYNNIKDADAAVQIVREFT-EPAAVAVKHMNPCGVGTGKTIAEAFDRAFEADKTSIFGGIIALNREVDKATAEALHNIFLEIIIAPSFSQEALDVLTAKKNLRLLTLDVSAA-V-QKEKQLTSVQGGLLIQDLDMHGFD-DAEISIPTKREPNEQEWEDLKLAWKVVKHVKSNAIVLAKDNMTVGVGAGQMNRVGSAKIAIEQAGE---KAKGSALGSDAYFPMPDTVEEAAKAGVTAIIQPGGSIRDEDSIKKADEYGIAMVFTGIRHFKH 4EHI Chain:A ((23-534)) -NAMRALLSVSDKEGIVEFGKELENLGFEILSTGGTFKLLKENGIKVIEVSDFT--------------------------------------ILGIDLVCVNLYPFKKTTIMS-DDFDEIIENIDIGGPAMIRSAAKNYKDVMVLCDPLDYEKVIETLKK-GQNDENFRLNLMIKAYEHTANYDAYIANYMNERFNGGFGASKFIVGQKVFDTKYGENPHQKGALYEF-DAF--FSANFKALKG-EASFNNLTDINAALNLASSFDKAPAIAIVKHGNPCGFAIKENLVQSYIHALKCDSVSAYGGVVAINGTLDEALANKINEIYVEVIIAANVDEKALAVFEGKKRIKIFTQESPFLIRSFDKYDFKHIDGGFVYQNSDEVGEDELKNAKLMSQREASKEELKDLEIAMKIAAFTKSNNVVYVKNGAMVAIGMGMTSRIDAAKAAIAKAKEMGLDLQGCVLASEAFFPFRDSIDEASKVGVKAIVEPGGSIRDDEVVKAADEYGMALYFTGVRHFLH

General information: TITO was launched using: RESULT: Template: 4EHI.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2387 -231965 -97.18 -496.71 target 2D structure prediction score : 0.67 Monomeric hydrophicity matching model chain A : 0.80 3D Compatibility (PKB) : -97.18 2D Compatibility (Sec. Struct. Predict.) : 0.67 1D Compatibility (Hydrophobicity) : 0.80 QMean score : 0.449

(partial model without unconserved sides chains): PDB file : Tito_4EHI.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4EHI-query.scw PDB file : Tito_Scwrl_4EHI.pdb: