Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ---------MNCWKTINLMKDYGAV---RIILT--AVCFMILVFISTFLAFELLRPGTSLSDEYVSLFGGLLVVILFVH---KVIHVLPIICKKRKIEKKFYILRMRTWKRIPKTTMLISLVSPFLL----------ITPVLFYAALAFPNHAHYFCMISGIHAGYCLPDFLL-ALKLIKAPKTAFIDQEADGLDILVEK 1GY1 Chain:A ((2-155)) TLDTTWKEATLPQVKAMLQKDTGKVSGDTVTYSGKTVHVVAAAVLP-GFPFPSFEVHDKKNPTLQIPAGA-TVDVTFINTNKGFGHDFDITK----KGPPYAVMPVID----------PIVAGTGFSPVPKDGKFGYTNFTWHP--TA--GTYYYVCQIPGHAATGMFGKIVVK--------------------------

General information: TITO was launched using: RESULT: Template: 1GY1.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 690 -133852 -193.99 -1062.31 target 2D structure prediction score : 0.24 Monomeric hydrophicity matching model chain A : 0.63 3D Compatibility (PKB) : -193.99 2D Compatibility (Sec. Struct. Predict.) : 0.24 1D Compatibility (Hydrophobicity) : 0.63 QMean score : -0.097

(partial model without unconserved sides chains): PDB file : Tito_1GY1.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1GY1-query.scw PDB file : Tito_Scwrl_1GY1.pdb: