Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIPLHENLAGKTAVITGGSGVLCSAMARELARHGMKVAILNRTAEKGQAVVKEITAAGGTACAVAADVLDRMSLERAKEDILGQFGAVDLLINGAGGNHPDAITDVETYEEAGEGQSFFDMDERGFLTVFSTNFTGAFLASQVFGKELLKADSPAIINLSSMSAYSPMTKVPAYSAAKASINNFTMWMAVHFAETGLRVNAIAPGFFLTKQNHDLLINQDGTFTSRS-HKIIAGTPMKRFGKPEDLLGTLLWLADESYSGFVTGITVPVDGGFMAYSGV 4NBW Chain:C ((6-257)) ----------RVAIITGAANGIGRATALEFAQAGYHVVAWDLAEEAGAALIAEIADAGGSADFARVDVSAAESVEAAVAEIIAEHGRVDVLVNNAGILHDGQLVKVKG------GEVVKKMAEAQFDAVISVNLKGVFLCTQAVAPHMIAAKYGRILNASSVVGLYGNFGQTNYVAAKSGVIGMTKVWARELGRYGITVNAIAPGFIATE-----MVQQ---MPERVLEAMVARTPVGRIGDPVDIARAYLFLASEE-SGFISGTTLSVDGGMVVGS--

General information: TITO was launched using: RESULT: Template: 4NBW.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 1354 -156280 -115.42 -622.63 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain C : 0.79 3D Compatibility (PKB) : -115.42 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.79 QMean score : 0.553

(partial model without unconserved sides chains): PDB file : Tito_4NBW.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4NBW-query.scw PDB file : Tito_Scwrl_4NBW.pdb: