Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLDPLDILTNIDDVLPYYQAIFSAEEQKVVGYEVLGRILAD-SEIQSLGPFFLDAGIPEEYKLEVDNRIIRQALDRFL-E---ADSDLLIFMNQDANLLMLDHGESFLELLKEYEA-KGIELHRFVLEITEHNFEGDIEQLYHMLAYYRTYGIKIAVDNIGKESSNLDRIALLSPDLLKIDLQALKVSQPSPSYEHVLYSISLLARKIGAALLYEDIEANFQLQYAWRNGGRYFQGYYLVSPSETFLERDVLKQRLKTEFHQFITHEKKKLETVYEHSEQFYKRVHQAVTSLRKNNLSSDDDFIKKLAEELTDCSFRIYMCDEEGDQLTGNVFKQDGEWIYQPEYAEKNWSWRPYFLENIMRMRNLRKGFFSDLYSDLETGEMIRTFSYPMDDQMYLFIDLPYSYLYEQDGLI 4Y9M Chain:A ((8-249)) -SSELRRALEANEFIPYYQPLSPGQGGRWIGVEVLMRWRHPREGLIRPDLFIPFAE-RSGLIVPMTRALMRQVAEDLGGHAGKLEPGFHIGFNISATHCHE---LALVDDCRELLAAFPPGHITLVLELTERELIESSEVTDRLFDELHALGVKIAIDDFGTGHSSLAYLRKFQVDCLKIDQSFVARIGIDTLSGHILDSIVELSAKLDLDIVAEGVETPEQRDYLAARGVDYLQGYLIGRPMPLES----------------------------------------------------------------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 4Y9M.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1130 -166515 -147.36 -705.57 target 2D structure prediction score : 0.53 Monomeric hydrophicity matching model chain A : 0.63 3D Compatibility (PKB) : -147.36 2D Compatibility (Sec. Struct. Predict.) : 0.53 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.410

(partial model without unconserved sides chains): PDB file : Tito_4Y9M.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4Y9M-query.scw PDB file : Tito_Scwrl_4Y9M.pdb: