Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSKIYVLDTNVLLQDPNAIFSFEENEVVIPAVVLEEVDSKKRYMDEVGRNARHVSKLIDALRQKGRLHEHVPLDTGGTLRIELNHRSFHQLQEIFIEKTNDNRILAVAKNLSLEEETKENGRPVILVSKDVLVRVKADAIGLLAEDFLNDRVVDNDEMYSGYKDLYISQQLFSSFYGKNQISVNDVKQHAFYPNQFALMKDELGGSSSAVGIADKTGTVLKRLVFDDEHIWGIRPKNVQQTMALELLLREDIPLVTLIGKAGTGKTLL-ALAAGLLQTED-LGIYKKLVVARPIVP-VGKDIGYLPGEKEEKLKPWMQPIFDNLEFLFNAKKPG------------E--------------LDAILAGIGSIQVEALTYIRGRSIPDQFIIIDEA-QNLTRHEVKTLLTRVG---EGSKIVL-MGDPEQIDHPYLDSLNNGLAYVVERFKGQPISGSVKLLKGERSGLAQLAADLL 5C78 Chain:A ((374-546)) ----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------KGEKIAFIGESGCGKSTLVDLIIGLLKPKEGQILIDKQELNASNAKNYRQKIGYIPQNI--YL--FNDSIAKNITFGDAVDEEKLNKVIKQANLEHFIKNLPQGVQTKVGDGGS-NLSGGQK--QRIAIARALYLEPEILVLDQATSALDTQSEAKIMDEIYKISKDKTMIIIAHRLSTI----------------------------------------------

General information: TITO was launched using: RESULT: Template: 5C78.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 492 -43517 -88.45 -313.07 target 2D structure prediction score : 0.52 Monomeric hydrophicity matching model chain A : 0.59 3D Compatibility (PKB) : -88.45 2D Compatibility (Sec. Struct. Predict.) : 0.52 1D Compatibility (Hydrophobicity) : 0.59 QMean score : 0.275

(partial model without unconserved sides chains): PDB file : Tito_5C78.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5C78-query.scw PDB file : Tito_Scwrl_5C78.pdb: