Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence --MRVISGSKKGRSLKAVAGTSTRPTTDKVKESIFNMIGPYFDGGRGLDLFAGSGGLGIEALSRGFEHCIFVD-RDFKAIQTVKSNLKTLELTKHAQVYRNDA-ERALHAAAKRETGFRGIFLDPPYKEQKLKALLTLIDEYQMLEEDGFIVAEHDREVELPETVGDLVMTRKETYGLTGVAIYKKRG- 2FPO Chain:D ((12-194)) GQIRIIGGQWRGRKLP----VPDSPGTDRVRETLFNWLAPVIVDAQCLDCFAGSGALGLEALSRYAAGATLIEMDRAVSQQLIKNLATLK--AGNARVVNSNAMSFLA----QKGTPHNIVFVDPPFRRGLLEETINLLEDNGWLADEALIYVESEVENGLPTVPANWSLHREKVAGQVAYRLYQREAQ

General information: TITO was launched using: RESULT: Template: 2FPO.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain D - contact count / total energy / energy per contact / energy per residue : 939 -79748 -84.93 -458.32 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain D : 0.78 3D Compatibility (PKB) : -84.93 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.78 QMean score : 0.524

(partial model without unconserved sides chains): PDB file : Tito_2FPO.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2FPO-query.scw PDB file : Tito_Scwrl_2FPO.pdb: