Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLEVKLPGLDLKNPIIPASGCFGFGKEFSRFYDLSCLGAIMIKATTKEPRFGNPTPRVAETGAGMLNAIGLQNPGLDSVLHHELPWLEQFDTPIIANVAGSQVDDYVEVAEHISKAPN---VHALELNISCPNVKTGGIAFGTNPEMAADLTKAVKEVSDVPVYVKLSPNV--ANITEIALAIEE-AGADGLTMINTLI-GMRLDLKTGKPIL--ANKTGGLSGPAVKPVAIRMVYEVSQMV-NIPIIGMGGVQTAEDALEFLLAGASAVAVGTANFV-NPFACPEIIEQLPSVLLQYGYQSIEECIGRSWNHEKQPAHHRA 4EF8 Chain:A ((36-346)) SLQVNLLNNTFANPFMNAAGVMCTTTEELVAMTESASGSLVSKSCTPALREGNPTPRYQALPLGSINSMGLPNNGFDFYLAYAAEQHDYGKKPLFLSMSGLSMRENVEMCKRLAAV-ATEKGVILELNLSCPNVPG-KPQVAYDFDAMRQCLTAVSEVYPHSFGVKMPPYFDFAHFDAAAEILNEFPKVQFITCINSIGNGLVIDAETESVVIKPKQGFGGLGGRYVLPTALANINAFYRRCPGKLIFGCGGVYTGEDAFLHVLAGASMVQVGTALQEEGPSIFERLTSELLGVMAKKRYQTLDEFRGKVRTL---------

General information: TITO was launched using: RESULT: Template: 4EF8.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1773 -198201 -111.79 -660.67 target 2D structure prediction score : 0.68 Monomeric hydrophicity matching model chain A : 0.74 3D Compatibility (PKB) : -111.79 2D Compatibility (Sec. Struct. Predict.) : 0.68 1D Compatibility (Hydrophobicity) : 0.74 QMean score : 0.496

(partial model without unconserved sides chains): PDB file : Tito_4EF8.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4EF8-query.scw PDB file : Tito_Scwrl_4EF8.pdb: