Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNQWMELANRVLAGAEVTDEEALSILHCP-DEDILLLMHGAFHIRKHFYGKKVKLNMIMNAKSGLCPENCGYCSQSAISKAPIESYRMVNKETLLEGAKRAHDLNIGTYCIVASGRGPS-NREVDQVVDAVQEIKETYGLKVCACLGLLKPEQAKRLKDAGVDRYNHNLNTS-QRNHSNITTSHTYDDRVNTVEIAKESGLSPCSGAIIGIKETKQDVIDIAKSLKALDADSIPVNFLHAIDGTPLEGVNELNPLYCLKVLALFRFINPSKEIRISGGREVNLRSLQPLGLY-AANSIFV-----------GDYL---TTAGQEETEDHKMLSDLGFEVESVEEMKASLSAKS 3T7V Chain:A ((12-348)) -----SLGDKVIEGYQLTDNDLRTLLSLESKEGLERLYSAARKVRDHYFGNRVFLNCFIYF-STYCKNQCSFCYYNCRNE---INRYRLTMEEIKETCKTLKGAGFHMVDLT-MGEDPYYYEDPNRFVELVQIVKEELGLPIMISPGLMDNATLLKAREKGANFLALYQETYDTELYRKLRVGQSFDGRVNARRFAKQQGYCVEDGILTGVGNDIESTILSLRGMSTNDPDMVRVMTFLPQEGTPLEGFRDKSNLSELKIISVLRLMFPKRLIPASLDLEGI--DGMVLRLNAGANIVTSILPPDSQLEGVANYDRDLEERDRDIKSVVRRLEIMGMKPARQADFEAVL----

General information: TITO was launched using: RESULT: Template: 3T7V.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1729 -168140 -97.25 -527.08 target 2D structure prediction score : 0.67 Monomeric hydrophicity matching model chain A : 0.69 3D Compatibility (PKB) : -97.25 2D Compatibility (Sec. Struct. Predict.) : 0.67 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.449

(partial model without unconserved sides chains): PDB file : Tito_3T7V.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3T7V-query.scw PDB file : Tito_Scwrl_3T7V.pdb: