Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MFKFKKNFLVGLSAALMSISLFSATASAASTDYWQNWTDGGGIVNAVNGSGGNYSVNWSNTGNFVVGKGWTTGSPFRTINYNAGVWAPNGNGYLTLYGWTRSPLIEYYVVDSWGTYRPTGTYKGTVKSDGGTYDIYTTTRYNAPSIDGDRTTFTQYWSVRQSKRPTGSNATITFSNHVNAWKSHGMNLGSNWAYQVMATEGYQSSGSSNVTVW 1M4W Chain:A ((20-179)) ------------------------------------WTDAPGTVSMTLHSGGSYSTSWRNTGNFVAGKGWSTGGR-RTVTYNAS-FNPSGNAYLTLYGWTRNPLVEYYIVESWGTYRPTGTYKGTVTTDGGTYDIYETWRYNAPSIEGTR-TFQQFWSVRQQKRTSG---TITIGNHFDAWARAGMNLGSH-DYQIMATEGYQ----------

General information: TITO was launched using: RESULT: Template: 1M4W.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 833 -32676 -39.23 -204.22 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain A : 0.79 3D Compatibility (PKB) : -39.23 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.79 QMean score : 0.575

(partial model without unconserved sides chains): PDB file : Tito_1M4W.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1M4W-query.scw PDB file : Tito_Scwrl_1M4W.pdb: