Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MESKVVENRLKEAKLIAVIRSKDKQEACQQIESLLDKGIRAVEVTYTTPGASDIIESF-RNREDILIGAGTVISAQQAGEAAKAGAQFIVSPGFSADLAEHLSFVKTHYIPGVLTPSEIMEALTFGFTTLKLFPSGVF-GIPFMKNLAGPFPQVTFIPTGGIHPSEVPDWLRAGAGAVGVGSQLGSCSKEDLQAVFQV 1FWR Chain:B ((27-173)) ------------------------EHAVPMAKALVAGGVRVLEVTLRTECAVDAIRAIAKEVPEAIVGAGTVLNPQQLAEVTEAGAQFAISPGLTEPLLKAATEGTIPLIPGISTVSELMLGMDYGLKEFQFFPAEANGGVKALQAIAGPFSQVRFCPKGGISPANYRDYL---------------------------

General information: TITO was launched using: RESULT: Template: 1FWR.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 630 -30461 -48.35 -210.08 target 2D structure prediction score : 0.70 Monomeric hydrophicity matching model chain B : 0.75 3D Compatibility (PKB) : -48.35 2D Compatibility (Sec. Struct. Predict.) : 0.70 1D Compatibility (Hydrophobicity) : 0.75 QMean score : 0.613

(partial model without unconserved sides chains): PDB file : Tito_1FWR.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1FWR-query.scw PDB file : Tito_Scwrl_1FWR.pdb: