Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNKTELINAVAEA-SELSKKDATKAVDSVFDTILDALKNGDKIQLIGFGNFEVRERSARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVAGK 1OWF Chain:B ((1-92)) MTKSELIERLATQQSHIPAKTVEDAVKEMLEHMASTLAQGERIAIRGFGSFSLHYRAPRTGRNPKTGDKVELEGKYVPHFKPGKELRDRANI-

General information: TITO was launched using: RESULT: Template: 1OWF.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 191 17428 91.25 191.52 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain B : 0.83 3D Compatibility (PKB) : 91.25 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.83 QMean score : 0.549

(partial model without unconserved sides chains): PDB file : Tito_1OWF.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1OWF-query.scw PDB file : Tito_Scwrl_1OWF.pdb: