Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKNEMHLEFSALSQNESFARVTVASFIAQLDPTMDELTEIKTVVSEAVTNAIIHGYEENCEGKVYISVTLEDHVVYMTIRDEGLGITD--LEEARQPLFTTKPELERSGMGFTIMENFMD----DVSIDSSPEMGTTIRLTKHLSKSKALCN 4BIX Chain:A ((188-296)) ------------------------------------PNALESALENIVRNALRYSHT-----KIEVGFAVDKDGITITVDDDGPGVSPEDREQIFRPFYRTD----GTGLGLAIVETAIQQHRGWVKAEDSPLGGLRLVIWLPLYK------

General information: TITO was launched using: RESULT: Template: 4BIX.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 407 -5410 -13.29 -56.94 target 2D structure prediction score : 0.73 Monomeric hydrophicity matching model chain A : 0.70 3D Compatibility (PKB) : -13.29 2D Compatibility (Sec. Struct. Predict.) : 0.73 1D Compatibility (Hydrophobicity) : 0.70 QMean score : 0.580

(partial model without unconserved sides chains): PDB file : Tito_4BIX.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4BIX-query.scw PDB file : Tito_Scwrl_4BIX.pdb: