Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKSLEEKTIAKEQIFSGKV-IDLYVEDVELPNGKAS---KREIVKHPGAVAVLAVTD--EGKIIMVKQFRKPLERT-----IVEIPAGKLEKGEEPEYTALRELEEETGYTAKKLTKITAFYTSPGFADEIVHVFLAE-ELSVLEEKREL-DEDEFVEVMEVTLEDALKLVESREVYDAKTAYAIQYLQLKEALQAQK 1GA7 Chain:B ((19-201)) --------IARETLYRGFFSLDLYRFRHRLFNGQMSHEVRREIFER-GHAAVLLPFDPVRDEVVLIEQIRIAAYDTSETPWLLEMVAGMIEEGESVEDVARREAIEEAGLIVKRTKPVLSFLASPGGTSERSSIMVGEVDATTASGIHGLADENEDIRVHVVSREQAYQWVEEGKIDNAASVIALQWLQLHH------

General information: TITO was launched using: RESULT: Template: 1GA7.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 712 43303 60.82 254.72 target 2D structure prediction score : 0.54 Monomeric hydrophicity matching model chain B : 0.76 3D Compatibility (PKB) : 60.82 2D Compatibility (Sec. Struct. Predict.) : 0.54 1D Compatibility (Hydrophobicity) : 0.76 QMean score : 0.443

(partial model without unconserved sides chains): PDB file : Tito_1GA7.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1GA7-query.scw PDB file : Tito_Scwrl_1GA7.pdb: