Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MARKLFTPITIKDMTLKNRIVMSPMCMYSSHEKDGKLTPFHMAHYISRAIGQVGLIIVEASAVNPQGRITDQDLGIWSDEHIEGFAKLTEQVKEQGSKIGIQLAHAGRKAE----LEGD------------IFAPSAIAFDEQ-SATPVEMSAEKVKETVQEFKQAAARAKEAGFDVIEIHAAHGYLIHEFLSPLSNHRTDEYGGSPENRYRFLREIIDEVKQVW--DGPLFVRVSASDYTDKG-LDIADHIGFAKWMKEQGVDLIDCSSG-ALVHADINVFPGYQVSFAEKIREQADMATGAVGMITDGSMAEEILQNGRADLIFIGRELLRDPFFARTAAKQLNTE-----IPAPVQYERGW 4UTM Chain:A ((6-359)) ----LFEPYTLKDVTLRNRIAIPPMCQYMA--EDGMINDWHHVHLAGLARGGAGLLVVEATAVAPEGRITPGCAGIWSDAHAQAFVPVVQAIKAAGSVPGIQIAHAGRKASANRPWEGDDHIAADDTRGWETIAPSAIAFGAHLPKVPREMTLDDIARVKQDFVDAARRARDAGFEWIELHFAHGFLGQSFFSEHSNKRTDAYGGSFDNRSRFLLETLAAVREVWPENLPLTARFGVLEYDGRDEQTLEESIELARRFKAGGLDLLSVSVGFTIPDTNIPWGPAFMGPIAERVRREAKLPVTSAWGFGTPQLAEAALQANQLDLVSVGRAHLADPHWAYFAAKELGVEKASWTLPAPYAH----

General information: TITO was launched using: RESULT: Template: 4UTM.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1990 -8837 -4.44 -26.94 target 2D structure prediction score : 0.77 Monomeric hydrophicity matching model chain A : 0.80 3D Compatibility (PKB) : -4.44 2D Compatibility (Sec. Struct. Predict.) : 0.77 1D Compatibility (Hydrophobicity) : 0.80 QMean score : 0.704

(partial model without unconserved sides chains): PDB file : Tito_4UTM.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4UTM-query.scw PDB file : Tito_Scwrl_4UTM.pdb: