Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNEKNIKHSQNFITSKHNIDKIMTNIRLNEHDNIFEIGSGKGHFTLELVQRCNFVTAIEIDHKLCKTTENKLVDH-DNFQVLNKDILQFKFPK---NQSYKIFGNIPYNISTDIIRKIVFDSI-ADEIYLIVEYGFAKRLLN--T---KRSLALFLMAEVDISILSMVPREYFHPKPKVNSSLIRLNRKKS-RISHKDKQKYNYFVMKWVNKE-------YKKIFTKNQFNNSLKHAGI---DDLNNISFEQFLSLFNSYKLFNK 3TQS Chain:A ((12-258)) ---------QHFLHDSFVLQKIVSAIHPQKTDTLVEIGPGRGALTDYLLTECDNLALVEIDRDLVAFLQKKY-NQQKNITIYQNDALQFDFSSVKTDKPLRVVGNLPYNISTPLLFHLFSQIHCIEDMHFMLQKEVVRRITAEVGSHDYGRLSVMAQYFCDNTYLFTVSPQAFTPPPRVESAIIRLIPRHNFTPVAKNLDQLSHVVKEAFSYRRKTVGNALKKLINPSQWP----LLEINPQLRPQELTVEDFVKISNILN----

General information: TITO was launched using: RESULT: Template: 3TQS.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1034 -21745 -21.03 -96.22 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain A : 0.77 3D Compatibility (PKB) : -21.03 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.77 QMean score : 0.570

(partial model without unconserved sides chains): PDB file : Tito_3TQS.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3TQS-query.scw PDB file : Tito_Scwrl_3TQS.pdb: